What does F4 80 stain for?

The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen.

What are F4 80 cells?

F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin.

Do T cells express F4 80?

F4/80 expression on Langerhans’ cells decreases after they take up antigens and become migrating DCs; in lymph nodes and spleens, F4/80 is detected only on macrophages in T cell–independent areas (7). These studies have pointed to a function for F4/80 in selected tissue macrophage populations.

What is Ly6C high?

The Ly6Chigh and Ly6Cmiddle subsets perform pro-inflammatory functions and express high level of CCR2, which is considered the counterpart of human classical MCs.

Can a F4 / 80 antibody be used in functional studies?

A low endotoxin version of the CI:A3-1 F4/80 antibody is available for use in functional studies. An anti-idiotypic CI:A31 antibody, clone 17867, is also available from Bio-Rad which is designed as an IHC control antibody for use in conjunction with clone Cl:A3-1.

Which is the best F4 / 80 antibody for microglia?

F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best: High specificity – shown to be highly targeted for all mouse macrophages. Added versatility for microglial cells – unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research.

Which is the mouse macrophage marker F4 / 80?

F4/80 antibody clone CI:A3-1 (MCA497), is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).

Which is part of the F4 / 80 glycoprotein family?

Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).