What is a reporter vector?

Reporter vectors contain a defining reporter gene, which can be used to detect the successful transformation of a gene of interest into a cell, as well the protein expression of that gene. Common reporters include fluorescence proteins (like GFP and RFP) or enzymes such as beta-galactosidase and luciferase.

What is luciferase plasmid?

Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.

What is reporter gene give example?

For example, yeast reporter genes include CUP1, a gene that enables yeast to grow on copper-containing media, URA3, a gene that kills yeast when growing on 5-fluorouracil, and ADE1 and ADE2, two genes that synthesize adenine.

What is reporter gene with example?

Reporter genes are those genes that when introduced into target cells (e.g., brain tissues, cancer, and circulating white cells) produce a protein receptor or enzyme that binds, transports, or traps a subsequently injected imaging probe.

Is lacZ a reporter gene?

The bacterial lacZ gene, which encodes the β-Gal enzyme, is the most commonly used reporter gene, because of its historical availability and the existence of a large panel of chromogenic8, 9 and fluorogenic10, 11, 12, 13 substrates.

Is luciferase better than GFP?

Bioluminescent luciferase imaging was shown to be more sensitive than fluorescent GFP imaging. Both bioluminescent and fluorescent intensity correlated significantly and linearly with tumor volume and tumor weight, as measured by caliper.

What are the PGL3 luciferase reporter vectors used for?

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis – or trans -acting factors.

How are TCF / LEF sites upstream of a luciferase reporter?

TCF/LEF sites upstream of a luciferase reporter. HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells. Control for beta-catenin reporter.

Which is the splicing reporter for the luciferase gene?

CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE: A splicing reporter that contains an intron in the middle of the Firefly luciferase gene. Learn more about CMV-LUC2CP/intron/ARE and CMV-LUC2CP/ARE in our 28 Hot Plasmids from 2015 blog post.

How are MCH and Luc dual reporter vectors used?

HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells. Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.