How much does Akta cost?

akta starts are around 15k, pures are 45-100 depending on the options.

What is an Akta system?

ÄKTA go is a small and compact liquid chromatography system that allows researchers to perform routine protein purification with ease while allowing for efficient use of bench and cold cabinet space.

How does an Akta work?

In Fast Protein Liquid Chromatography (FPLC), a machine like the AKTA takes our protein sample and pumps it onto a column (which it’s gotten ready by flowing a bunch of buffer (pH-stable salt water) to “equilibrate” it. It then washes the column with the buffers we tell it to.

How do you use Akta?

Akta Pure General Protocol V. 1

1. Connect buffers and prime pumps. Complete a pump wash with water on all pumps (A and B).
2. Connect column. In Unicorn, go to “Manual Instructions” > “Alarms” > “Alarm pre column pressure.” Set the high alarm pressure limit that is appropriate for the column.
3. Prime and fill sample loop.

What is Akta pilot?

ÄKTA pilot 600 is a bench-top chromatography system with a wide flow and pressure range. It suits both production of technical batches and scale-up studies as well as small-scale production of GMP-grade material. Match product yield to project needs.

How do you inject Akta pure?

1. Fill a syringe with at least 2x the amount of water as the size of the sample loop.
2. Repeat with your starting buffer.
3. Set the injection valve to “inject.” Remove the syringe and connect a syringe with your sample to the syringe port.

What’s the maximum pressure for Akta pure 150?

The maximum pressure for Mixer chamber 15 ml is 5 MPa. Four different Mixer chambers are available for ÄKTA pure 150. Their volumes are: 0.6 ml, 1.4 ml

What is the model ID of Akta avant 150?

ÄKTA avant 150 ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes. Change Product Select Model ID Product Name 28976337

What should I know about the Akta pure system?

See ÄKTA pure System Handbook, chapter Prepare the system for a run. 1 PEEK tubing is biocompatible and chemically inert to most solvents used in the purification of proteins. It has in general very good pressure limits, especially for water based buffers.

What is the flow rate of Akta chromatography?

ÄKTA pure chromatography system allows for quick and easy customization thanks to the modular design of both the hardware and software. Choose from ÄKTA pure 25 with a flow rate up to 25 mL/min or ÄKTA pure 150 with a flow rate up to 150 mL/min and combine with more than 20 hardware options.